Genomic sites hypersensitive to ultraviolet radiation

uvhl, an Arabidopsis Mutant Hypersensitive to Ultraviolet Light and lonizing Radiation

A genetic screen for mutants of Arabidopsis that are hypersensitive to UV light was developed and used to isolate a new mutant designated uvhl. UV hypersensitivity in uvhl was due to a single recessive trait that is probably located on chromosome 3. Although isolated as hypersensitive to an acute exposure to UV-C light, uvhl was also hypersensitive to UV-B wavelengths, which are present in sunl...

Current bioinformatic approaches to identify DNase I hypersensitive sites and genomic footprints from DNase-seq data

CHROMATIN ACCESSIBILITY The formation of regions of open chromatin or nucleosome loss in eukaryotic genomes is an important factor elucidating potential regulatory activity. Nucleosome packaging, which organizes the DNA structure, acts as a regulator of transcription by enabling or restricting protein binding, and therefore facilitating the replication and coordination of gene activity (Cockeri...

Diurnal variations in solar ultraviolet radiation at typical anatomical sites.

OBJECTIVE Solar ultraviolet (UV) radiation is an important environmental factor that affects human health. The understanding of diurnal variations of UV radiation at anatomical sites may be helpful in developing ways to protect humans from the harmful effects of UV radiation. METHODS In order to characterize the diurnal variations, the UV exposure values were measured at 30 min intervals by u...

Systemic Response to Ultraviolet Radiation

Native genomic blotting: high-resolution mapping of DNase I-hypersensitive sites and protein-DNA interactions.

DNase I-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. We have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic DNA on native polyacrylamide gels prior to electroblotting and hybridization. This improved method has been used to scan th...